Hotspot

ccsA-ndhD

SSC · 116672-116926 · 255 bp

Back to Coluria

Pi 0.0745

MarkerSeek score 69.80

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 255
variable_sites: 19
pi: 0.0745
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ATGCAGCTCTTTTGTGTGGA	TGGACCGCGAGAGTTATTTG	45.0	50.0	58.0	58.0	921-927 bp	1.0	81.8
ccsA-ndhD_p2	TGCAGCTCTTTTGTGTGGAT	TGGACCGCGAGAGTTATTTG	45.0	50.0	58.0	58.0	920-926 bp	1.0	81.8
ccsA-ndhD_p3	ATGCAGCTCTTTTGTGTGGA	GGACCGCGAGAGTTATTTGT	45.0	50.0	58.0	58.0	920-926 bp	1.0	81.7
ccsA-ndhD_p4	TGCAGCTCTTTTGTGTGGAT	GGACCGCGAGAGTTATTTGT	45.0	50.0	58.0	58.0	919-925 bp	1.0	81.7
ccsA-ndhD_p5	TGCAGCTCTTTTGTGTGGAT	CTCGACGAAATGGGTGGAAT	45.0	50.0	58.0	58.0	1210-1216 bp	1.0	81.3