Hotspot

rpl14-rpl16

LSC · 82028-82179 · 152 bp

Back to Coluria

Pi 0.0728

MarkerSeek score 70.00

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 152
variable_sites: 11
pi: 0.0728
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	TCGGATCTTTCTAGAGGCGT	GTTAAACCCGGCCGAATACT	50.0	50.0	57.9	58.0	442-449 bp	1.0	82.0
rpl14-rpl16_p2	TCGGATCTTTCTAGAGGCGT	CGTCGTGGCGGAAAAATATG	50.0	50.0	57.9	57.9	556-563 bp	1.0	81.3
rpl14-rpl16_p3	TCGGATCTTTCTAGAGGCGT	AGAAACACGTATGGGTTCGG	50.0	50.0	57.9	57.9	494-501 bp	1.0	81.3
rpl14-rpl16_p4	TCGGATCTTTCTAGAGGCGT	TATGGGTTCGGGGAAAGGAT	50.0	50.0	57.9	58.1	485-492 bp	1.0	81.2
rpl14-rpl16_p5	TGTCTTAATTCCCGGGGGAT	GTTAAACCCGGCCGAATACT	50.0	50.0	58.1	58.0	598-605 bp	1.0	81.0