Hotspot

psbZ-trnG-GCC

LSC · 36673-37085 · 413 bp

Back to Coluria

Pi 0.0584

MarkerSeek score 77.70

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 413
variable_sites: 24
pi: 0.0584
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTTGCTTCTCCTGAGGGTTG	AATCGAACCCGCATCTTCTC	50.0	50.0	57.7	58.1	582-683 bp	1.0	80.6
psbZ-trnG-GCC_p2	TTTGCTTCTCCTGAGGGTTG	GAATCGAACCCGCATCTTCT	50.0	50.0	57.7	58.1	583-684 bp	1.0	80.6
psbZ-trnG-GCC_p3	GGGTTGGTCGGGTAACAAAA	AATCGAACCCGCATCTTCTC	50.0	50.0	58.3	58.1	568-669 bp	1.0	80.4
psbZ-trnG-GCC_p4	GGGTTGGTCGGGTAACAAAA	GAATCGAACCCGCATCTTCT	50.0	50.0	58.3	58.1	569-670 bp	1.0	80.4
psbZ-trnG-GCC_p5	TTTGCTTCTCCTGAGGGTTG	ACTCATTATCTGGGGCGGAT	50.0	50.0	57.7	58.3	608-709 bp	1.0	78.8