Hotspot

petN-psbM

LSC · 28432-29652 · 1221 bp

Back to Coluria

Pi 0.04

MarkerSeek score 69.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1221
variable_sites: 48
pi: 0.04
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTAAATCTCGCTTGGGCTG	GTGGTAAGTGTAAGTGCGGT	50.0	50.0	58.0	57.8	1773-1819 bp	1.0	82.4
petN-psbM_p2	AAGTTAATGAAGGGCCGCAA	GTGGTAAGTGTAAGTGCGGT	45.0	50.0	58.1	57.8	2082-2128 bp	1.0	81.6
petN-psbM_p3	AGTTAATGAAGGGCCGCAAA	GTGGTAAGTGTAAGTGCGGT	45.0	50.0	58.1	57.8	2081-2127 bp	1.0	81.6
petN-psbM_p4	TTTCCCCAGTTCAAATCCGG	GTGGTAAGTGTAAGTGCGGT	50.0	50.0	58.1	57.8	2167-2223 bp	1.0	81.6
petN-psbM_p5	GTATCATTTTGGCGGCATGG	GTGGTAAGTGTAAGTGCGGT	50.0	50.0	57.9	57.8	2219-2275 bp	1.0	81.1