Hotspot

trnS-GCU-trnG-UCC

LSC · 8571-9213 · 643 bp

Back to Coluria

Pi 0.043
MarkerSeek score 81.30
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
643
variable_sites
25
pi
0.043
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA AATCAAACCGAAAGACCCTT 50.0 40.0 58.0 55.1 694-754 bp 1.0 63.9
trnS-GCU-trnG-UCC_p2 CAATCCGACGCTTTAGTCCA ATCAAACCGAAAGACCCTTT 50.0 40.0 58.0 55.1 693-753 bp 1.0 63.9
trnS-GCU-trnG-UCC_p3 AATCCGACGCTTTAGTCCAC AATCAAACCGAAAGACCCTT 50.0 40.0 58.0 55.1 693-753 bp 1.0 63.9
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC ATCAAACCGAAAGACCCTTT 50.0 40.0 58.0 55.1 692-752 bp 1.0 63.9
trnS-GCU-trnG-UCC_p5 ACGGAAAGAGAGGGATTCGA ATCAAACCGAAAGACCCTTT 50.0 40.0 58.2 55.1 747-807 bp 1.0 62.6