Hotspot

psbK-psbI

LSC · 7974-8204 · 231 bp

Back to Coluria

Pi 0.0478

MarkerSeek score 70.00

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 231
variable_sites: 11
pi: 0.0478
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	ATGGCAAGCTGCTGTAAGTT	ACAAAGAATATCACTACCGTGT	45.0	36.4	58.1	55.2	306-315 bp	1.0	44.1
psbK-psbI_p2	TGGCAAGCTGCTGTAAGTTT	ACAAAGAATATCACTACCGTGT	45.0	36.4	58.3	55.2	305-314 bp	1.0	43.1
psbK-psbI_p3	ATGGCAAGCTGCTGTAAGTT	AACAAAGAATATCACTACCGTGT	45.0	34.8	58.1	56.0	307-316 bp	1.0	43.1
psbK-psbI_p4	CTTTGTATGGCAAGCTGCTG	ACAAAGAATATCACTACCGTGT	50.0	36.4	57.7	55.2	312-321 bp	1.0	43.0
psbK-psbI_p5	TTTGTATGGCAAGCTGCTGT	ACAAAGAATATCACTACCGTGT	45.0	36.4	58.4	55.2	311-320 bp	1.0	43.0