Hotspot

rps16-trnQ-UUG

LSC · 6458-7302 · 845 bp

Back to Coluria

Pi 0.0463

MarkerSeek score 79.50

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 845
variable_sites: 38
pi: 0.0463
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CTCCACAGCTGATCATGTCC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.0	58.1	941-1020 bp	1.0	84.1
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	918-997 bp	1.0	83.5
rps16-trnQ-UUG_p3	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	929-1008 bp	1.0	83.0
rps16-trnQ-UUG_p4	CCACAGCTGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	939-1018 bp	1.0	81.2
rps16-trnQ-UUG_p5	TCCACAGCTGATCATGTCCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.4	58.1	940-1019 bp	1.0	81.1