Hotspot

trnK-UUU-rps16

LSC · 4306-5322 · 1017 bp

Back to Coluria

Pi 0.0565
MarkerSeek score 81.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1017
variable_sites
56
pi
0.0565
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GGGCAGGGGTTTTTAAGGAA 50.0 50.0 58.2 58.0 1089-1150 bp 1.0 84.4
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT GGGCAGGGGTTTTTAAGGAA 50.0 50.0 58.2 58.0 1088-1149 bp 1.0 84.4
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT AAGGGCAGGGGTTTTTAAGG 50.0 50.0 58.2 58.0 1091-1152 bp 1.0 84.3
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT AAGGGCAGGGGTTTTTAAGG 50.0 50.0 58.2 58.0 1090-1151 bp 1.0 84.3
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 1133-1194 bp 1.0 84.1