Hotspot

rpoC2

LSC · 16839-21029 · 4191 bp

Back to Colquhounia

Pi 0.00138

MarkerSeek score 47.30

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4191
variable_sites: 14
pi: 0.00138
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	CCGAATCAAATGACTGCACC	GGGTCGAATCGATTGGTTGA	50.0	50.0	57.5	58.0	323 bp	1.0	75.9
rpoC2_p2	CCGAATCAAATGACTGCACC	AGGCCTGAAAGAGAACGTTG	50.0	50.0	57.5	58.1	302 bp	1.0	75.2
rpoC2_p3	CCGAATCAAATGACTGCACC	AGAGAACGTTGTTTTGGGGG	50.0	50.0	57.5	58.3	293 bp	1.0	73.6
rpoC2_p4	CCGAATCAAATGACTGCACC	GGCCTGAAAGAGAACGTTGT	50.0	50.0	57.5	58.4	301 bp	1.0	72.9
rpoC2_p5	CCGAATCAAATGACTGCACC	CGGATTGAAAGGATTCGTGC	50.0	50.0	57.5	57.5	254 bp	1.0	72.6