Hotspot

clpP-psbB

LSC · 71207-71643 · 437 bp

Back to Colquhounia

Pi 0.0389

MarkerSeek score 68.20

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 437
variable_sites: 41
pi: 0.0389
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	525-539 bp	1.0	87.7
clpP-psbB_p2	CGCACTATACGTCAACCCAA	GGCCGGGATCATTCAATACA	50.0	50.0	58.0	57.7	557-571 bp	1.0	86.9
clpP-psbB_p3	CGCACTATACGTCAACCCAA	ACACGATACCAAGGCAAACC	50.0	50.0	58.0	58.5	528-542 bp	1.0	85.8
clpP-psbB_p4	CGTCAACCCAAGATGCATCT	CGATACCAAGGCAAACCCAT	50.0	50.0	58.3	58.2	516-530 bp	1.0	85.8
clpP-psbB_p5	CGTCAACCCAAGATGCATCT	GGCCGGGATCATTCAATACA	50.0	50.0	58.3	57.7	548-562 bp	1.0	85.0