Hotspot

petA-psbJ

LSC · 62975-64025 · 1051 bp

Back to Colquhounia

Pi 0.00504

MarkerSeek score 50.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1051
variable_sites: 13
pi: 0.00504
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GAGAAGGTTCAATTGGCCGA	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.2	57.5	1107-1108 bp	1.0	75.7
petA-psbJ_p2	TCGAGAAGGTTCAATTGGCC	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.2	57.5	1109-1110 bp	1.0	75.7
petA-psbJ_p3	GAGAAGGTTCAATTGGCCGA	CCGGATTAGGTTCATCCCTG	50.0	55.0	58.2	57.4	1106-1107 bp	1.0	75.2
petA-psbJ_p4	TCGAGAAGGTTCAATTGGCC	CCGGATTAGGTTCATCCCTG	50.0	55.0	58.2	57.4	1108-1109 bp	1.0	75.2
petA-psbJ_p5	AGAAGGTTCAATTGGCCGAA	TCCGGATTAGGTTCATCCCT	45.0	50.0	57.7	57.5	1106-1107 bp	1.0	74.8