Hotspot

accD-psaI

LSC · 58766-59451 · 686 bp

Back to Colquhounia

Pi 0.00321

MarkerSeek score 49.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 686
variable_sites: 5
pi: 0.00321
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TAAGCTCCACGCCTTCTTTC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	802-803 bp	1.0	79.5
accD-psaI_p2	TAAGCTCCACGCCTTCTTTC	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.9	57.9	808-809 bp	1.0	78.9
accD-psaI_p3	CACGCCTTCTTTCCCTTGAA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.4	58.0	795-796 bp	1.0	77.1
accD-psaI_p4	CACGCCTTCTTTCCCTTGAA	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.4	57.9	801-802 bp	1.0	76.5
accD-psaI_p5	TAAGCTCCACGCCTTCTTTC	GCCGGAAATACTAGGCCTAC	50.0	55.0	57.9	57.5	791-792 bp	1.0	76.1