Hotspot

rbcL-accD

LSC · 56610-57298 · 689 bp

Back to Colquhounia

Pi 0.0029

MarkerSeek score 50.00

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 689
variable_sites: 4
pi: 0.0029
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CCGCTTGTGAGGTATGGAAA	ACCTGCGTTCTAACTCCTTC	50.0	50.0	57.9	57.3	821-822 bp	1.0	73.6
rbcL-accD_p2	CCGCTTGTGAGGTATGGAAA	TCCCATACCTGCGTTCTAAC	50.0	50.0	57.9	57.0	827-828 bp	1.0	71.7
rbcL-accD_p3	CCGCTTGTGAGGTATGGAAA	CCCATACCTGCGTTCTAACT	50.0	50.0	57.9	57.0	826-827 bp	1.0	71.7
rbcL-accD_p4	CCGCTTGTGAGGTATGGAAA	CCTGCGTTCTAACTCCTTCT	50.0	50.0	57.9	57.0	820-821 bp	1.0	71.2
rbcL-accD_p5	GCTTGCAAATGGAGTCCTGA	ACCTGCGTTCTAACTCCTTC	50.0	50.0	58.5	57.3	849-850 bp	1.0	70.7