Hotspot

atpB-rbcL

LSC · 54361-55163 · 803 bp

Back to Colquhounia

Pi 0.00399

MarkerSeek score 46.80

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 803
variable_sites: 8
pi: 0.00399
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GATGATACGCCCCTGGTTTT	AACACCCGCTTTGAATCCAA	50.0	45.0	57.9	58.3	932 bp	1.0	77.9
atpB-rbcL_p2	GGAAACCCCAGAACCAGAAG	AACACCCGCTTTGAATCCAA	55.0	45.0	58.1	58.3	899 bp	1.0	77.6
atpB-rbcL_p3	GGCGGAAAGGCTACATCTAG	AACACCCGCTTTGAATCCAA	55.0	45.0	57.8	58.3	970 bp	1.0	77.0
atpB-rbcL_p4	ATTTGGATGATACGCCCCTG	AACACCCGCTTTGAATCCAA	50.0	45.0	57.7	58.3	937 bp	1.0	76.1
atpB-rbcL_p5	GATGATACGCCCCTGGTTTT	TAACACCCGCTTTGAATCCA	50.0	45.0	57.9	57.4	933 bp	1.0	75.7