Hotspot

trnT(UGU)-trnL(UAA)

LSC · 46404-47096 · 693 bp

Back to Colquhounia

Pi 0.00734

MarkerSeek score 48.20

Primer available yes

Label trnT(UGU)-trnL(UAA)

Feature Metadata

label_name: trnT(UGU)-trnL(UAA)
feature_type: igs
region: LSC
length_bp: 693
variable_sites: 12
pi: 0.00734
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT(UGU)-trnL(UAA)_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	730-754 bp	1.0	80.8
trnT(UGU)-trnL(UAA)_p2	TAGGAAAAAGCCGGCTATCG	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	781-805 bp	1.0	79.8
trnT(UGU)-trnL(UAA)_p3	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	762-786 bp	1.0	79.8
trnT(UGU)-trnL(UAA)_p4	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	724-748 bp	1.0	79.6
trnT(UGU)-trnL(UAA)_p5	TAGGAAAAAGCCGGCTATCG	CGTCTACCGATTTCGCCATA	50.0	50.0	57.8	57.6	775-799 bp	1.0	78.5