Hotspot

psaA-ycf3

LSC · 41956-42683 · 728 bp

Back to Colquhounia

Pi 0.0103

MarkerSeek score 44.20

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 728
variable_sites: 18
pi: 0.0103
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CTTCTGGTTCCGGCGAAC	TTGAAGATCACGAGGCGTTT	61.1	45.0	58.4	57.8	776-783 bp	1.0	62.2
psaA-ycf3_p2	CTTCTGGTTCCGGCGAAC	TTGGTTGAAGATCACGAGGC	61.1	50.0	58.4	58.2	780-787 bp	1.0	61.9
psaA-ycf3_p3	CTTCTGGTTCCGGCGAAC	TGAAGATCACGAGGCGTTTC	61.1	50.0	58.4	58.3	775-782 bp	1.0	61.2
psaA-ycf3_p4	CTTCTGGTTCCGGCGAAC	TGGTTTGATCAAGCTGCTGA	61.1	45.0	58.4	57.7	854-861 bp	1.0	61.0
psaA-ycf3_p5	CTTCTGGTTCCGGCGAAC	TCACGAGGCGTTTCGAATAA	61.1	45.0	58.4	57.6	769-776 bp	1.0	60.7