Hotspot

trnK(UUU)-rps16

LSC · 4306-5076 · 771 bp

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Pi 0.00415
MarkerSeek score 48.10
Primer available yes
Label trnK(UUU)-rps16

Feature Metadata

label_name
trnK(UUU)-rps16
feature_type
igs
region
LSC
length_bp
771
variable_sites
8
pi
0.00415
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK(UUU)-rps16_p1 AAAGCCGAGTACTCTACCGT AGGAACTTCCGCCTAATCAA 50.0 45.0 58.2 56.5 840-995 bp 1.0 68.4
trnK(UUU)-rps16_p2 AAGCCGAGTACTCTACCGTT AGGAACTTCCGCCTAATCAA 50.0 45.0 58.2 56.5 839-994 bp 1.0 68.4
trnK(UUU)-rps16_p3 TCTAGCCGCACTTAAAAGCC AGGAACTTCCGCCTAATCAA 50.0 45.0 58.3 56.5 854-1009 bp 1.0 67.6
trnK(UUU)-rps16_p4 CCGCACTTAAAAGCCGAGTA AGGAACTTCCGCCTAATCAA 50.0 45.0 58.3 56.5 849-1004 bp 1.0 67.5
trnK(UUU)-rps16_p5 AAGCCGAGTACTCTACCGTT GTGCTCAACCCACAGGAAC 50.0 57.9 58.2 58.7 893-1048 bp 1.0 66.7