Hotspot

trnH(GUG)-psbA

LSC · 77-424 · 348 bp

Back to Colquhounia

Pi 0.0201
MarkerSeek score 57.90
Primer available yes
Label trnH(GUG)-psbA

Feature Metadata

label_name
trnH(GUG)-psbA
feature_type
igs
region
LSC
length_bp
348
variable_sites
14
pi
0.0201
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH(GUG)-psbA_p1 GATCCACTTGGCTACATCCG CAACCGTGCTAACCTTGGTA 55.0 50.0 58.1 57.8 467-548 bp 0.8 72.2
trnH(GUG)-psbA_p2 GATCCACTTGGCTACATCCG ATCAACCGTGCTAACCTTGG 55.0 50.0 58.1 58.2 469-550 bp 0.8 72.2
trnH(GUG)-psbA_p3 CAATCCACTGCCTTGATCCA CAACCGTGCTAACCTTGGTA 50.0 50.0 57.9 57.8 481-562 bp 0.8 72.1
trnH(GUG)-psbA_p4 AATCCACTGCCTTGATCCAC CAACCGTGCTAACCTTGGTA 50.0 50.0 57.9 57.8 480-561 bp 0.8 72.1
trnH(GUG)-psbA_p5 ACAATCCACTGCCTTGATCC CAACCGTGCTAACCTTGGTA 50.0 50.0 57.9 57.8 482-563 bp 0.8 72.1