Hotspot

atpF

LSC · 13672-15041 · 1370 bp

Back to Colocasia

Pi 0.00183

MarkerSeek score 60.90

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1370
variable_sites: 5
pi: 0.00183
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	ACTAATTTCGTCGGCTCGAA	TCAATCAAAAAGGGGAGGGC	45.0	50.0	57.4	58.1	1544-1554 bp	1.0	75.1
atpF_p2	ACTAATTTCGTCGGCTCGAA	GAGGGCGAAGTGATCCAAAA	45.0	50.0	57.4	58.2	1530-1540 bp	1.0	74.2
atpF_p3	ACTAATTTCGTCGGCTCGAA	AGGGCGAAGTGATCCAAAAA	45.0	45.0	57.4	57.7	1529-1539 bp	1.0	73.4
atpF_p4	AATTTCGTCGGCTCGAATGG	TCAATCAAAAAGGGGAGGGC	50.0	50.0	59.0	58.1	1541-1551 bp	1.0	72.3
atpF_p5	AATTTCGTCGGCTCGAATGG	GAGGGCGAAGTGATCCAAAA	50.0	50.0	59.0	58.2	1527-1537 bp	1.0	71.4