Hotspot

ndhD-psaC

SSC · 122952-123084 · 133 bp

Back to Colocasia

Pi 0.0677

MarkerSeek score 66.90

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 133
variable_sites: 18
pi: 0.0677
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AACCAAGGAAAATAACTCGT	GCATGAGACAACTCGCAGTA	35.0	50.0	52.7	58.0	190 bp	1.0	47.6
ndhD-psaC_p2	TGTTAACCAAGGAAAATAACTCGT	GCATGAGACAACTCGCAGTA	33.3	50.0	56.8	58.0	194 bp	1.0	47.4
ndhD-psaC_p3	AACCAAGGAAAATAACTCGT	TATGGCATGAGACAACTCGC	35.0	50.0	52.7	58.1	194 bp	1.0	47.4
ndhD-psaC_p4	TGTTAACCAAGGAAAATAACTCGT	TATGGCATGAGACAACTCGC	33.3	50.0	56.8	58.1	198 bp	1.0	47.3
ndhD-psaC_p5	TGTTAACCAAGGAAAATAACTCGT	AACCGAGGACTGTGTAGGTT	33.3	50.0	56.8	58.3	272 bp	1.0	45.3