Hotspot

petA-psbJ

LSC · 68135-69030 · 896 bp

Back to Colocasia

Pi 0.00502

MarkerSeek score 62.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 896
variable_sites: 9
pi: 0.00502
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TCCGTTACGTGTTCAAGGTC	TCCGGATTAGGCTCATCTCT	50.0	50.0	57.9	57.3	1038 bp	1.0	74.1
petA-psbJ_p2	CCGTTACGTGTTCAAGGTCT	TCCGGATTAGGCTCATCTCT	50.0	50.0	57.9	57.3	1037 bp	1.0	74.1
petA-psbJ_p3	TCCGTTACGTGTTCAAGGTC	CCGGATTAGGCTCATCTCTG	50.0	55.0	57.9	57.3	1037 bp	1.0	73.7
petA-psbJ_p4	CCGTTACGTGTTCAAGGTCT	CCGGATTAGGCTCATCTCTG	50.0	55.0	57.9	57.3	1036 bp	1.0	73.6
petA-psbJ_p5	TCCGTTACGTGTTCAAGGTC	TTCCGGATTAGGCTCATCTCT	50.0	47.6	57.9	58.0	1039 bp	1.0	71.3