Hotspot

tRNA-Ser(GCU)-tRNA-Gly(UCC)

LSC · 9353-10921 · 1569 bp

Back to Colocasia

Pi 0.00931

MarkerSeek score 62.80

Primer available yes

Label tRNA-Ser(GCU)-tRNA-Gly(UCC)

Feature Metadata

label_name: tRNA-Ser(GCU)-tRNA-Gly(UCC)
feature_type: igs
region: LSC
length_bp: 1569
variable_sites: 27
pi: 0.00931
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Ser(GCU)-tRNA-Gly(UCC)_p1	GAGAAAGCGGAGAAAGAGGG	AGGACGAATCACACTTTTACCA	55.0	40.9	58.0	57.7	1679-1743 bp	1.0	66.4
tRNA-Ser(GCU)-tRNA-Gly(UCC)_p2	ATTAGCAATCCGCCGCTTTA	AGGACGAATCACACTTTTACCA	45.0	40.9	58.0	57.7	1624-1688 bp	1.0	66.2
tRNA-Ser(GCU)-tRNA-Gly(UCC)_p3	GAGAAAGCGGAGAAAGAGGG	GGACGAATCACACTTTTACCAC	55.0	45.5	58.0	57.6	1678-1742 bp	1.0	65.9
tRNA-Ser(GCU)-tRNA-Gly(UCC)_p4	ATTAGCAATCCGCCGCTTTA	GGACGAATCACACTTTTACCAC	45.0	45.5	58.0	57.6	1623-1687 bp	1.0	65.8
tRNA-Ser(GCU)-tRNA-Gly(UCC)_p5	TCAAGTGACCAGAGAAAGCG	AGGACGAATCACACTTTTACCA	50.0	40.9	57.8	57.7	1690-1754 bp	1.0	65.3