Hotspot

tRNA-Lys(UUU)-rps16

LSC · 4635-5392 · 758 bp

Back to Colocasia

Pi 0.00616

MarkerSeek score 67.50

Primer available yes

Label tRNA-Lys(UUU)-rps16

Feature Metadata

label_name: tRNA-Lys(UUU)-rps16
feature_type: igs
region: LSC
length_bp: 758
variable_sites: 9
pi: 0.00616
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Lys(UUU)-rps16_p1	AAAGCCGAGTACTCTACCGT	AAAGGAGCTCAACCCACAAG	50.0	50.0	58.2	58.0	836-855 bp	1.0	82.1
tRNA-Lys(UUU)-rps16_p2	AAGCCGAGTACTCTACCGTT	AAAGGAGCTCAACCCACAAG	50.0	50.0	58.2	58.0	835-854 bp	1.0	82.1
tRNA-Lys(UUU)-rps16_p3	AAAGCCGAGTACTCTACCGT	AGCTCAACCCACAAGAACTG	50.0	50.0	58.2	58.0	831-850 bp	1.0	82.0
tRNA-Lys(UUU)-rps16_p4	AAGCCGAGTACTCTACCGTT	AGCTCAACCCACAAGAACTG	50.0	50.0	58.2	58.0	830-849 bp	1.0	82.0
tRNA-Lys(UUU)-rps16_p5	CCGCACTTAAAAGCCGAGTA	AAAGGAGCTCAACCCACAAG	50.0	50.0	58.3	58.0	845-864 bp	1.0	81.2