Hotspot

clpP

LSC · 128323-130330 · 2008 bp

Back to Colobanthus

Pi 0.00437

MarkerSeek score 49.30

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2008
variable_sites: 22
pi: 0.00437
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	GGATTTCGTGACATGTCGGA	GATTATCGCGGAACAAGGGT	50.0	50.0	58.0	58.1	2530-2548 bp	1.0	81.4
clpP_p2	GGATTTCGTGACATGTCGGA	CCCTGTAATACTCCAACCGC	50.0	55.0	58.0	58.1	2865-2884 bp	1.0	81.3
clpP_p3	GGATTTCGTGACATGTCGGA	CCTGTAATACTCCAACCGCC	50.0	55.0	58.0	58.1	2864-2883 bp	1.0	81.3
clpP_p4	GGATTTCGTGACATGTCGGA	CTGTAATACTCCAACCGCCC	50.0	55.0	58.0	58.1	2863-2882 bp	1.0	81.3
clpP_p5	GGATTTCGTGACATGTCGGA	ACGAACATACCCTGTCTCCA	50.0	50.0	58.0	58.1	2804-2823 bp	1.0	81.2