Hotspot

trnL-CAA-trnL-CAA

LSC · 150531-150712 · 182 bp

Back to Colobanthus

Pi 0.0194

MarkerSeek score 59.90

Primer available yes

Label trnL-CAA-trnL-CAA

Feature Metadata

label_name: trnL-CAA-trnL-CAA
feature_type: igs
region: LSC
length_bp: 182
variable_sites: 9
pi: 0.0194
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-trnL-CAA_p1	CGAATGGCTCTTTCCGTTCT	TGCTAAAAAGCGTGGAGGTT	50.0	45.0	58.3	58.0	338 bp	1.0	80.0
trnL-CAA-trnL-CAA_p2	CGAATGGCTCTTTCCGTTCT	AAATGGTAGACACGCGAGAC	50.0	50.0	58.3	58.0	369 bp	1.0	79.9
trnL-CAA-trnL-CAA_p3	CGAATGGCTCTTTCCGTTCT	TGAAATGGTAGACACGCGAG	50.0	50.0	58.3	58.0	371 bp	1.0	79.9
trnL-CAA-trnL-CAA_p4	TCGAATGGCTCTTTCCGTTC	AAATGGTAGACACGCGAGAC	50.0	50.0	58.3	58.0	370 bp	1.0	79.9
trnL-CAA-trnL-CAA_p5	TCGAATGGCTCTTTCCGTTC	TGAAATGGTAGACACGCGAG	50.0	50.0	58.3	58.0	372 bp	1.0	79.9