Hotspot

petA-psbJ

LSC · 121031-121993 · 963 bp

Back to Colobanthus

Pi 0.00902
MarkerSeek score 48.40
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
963
variable_sites
21
pi
0.00902
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
48.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 ACGCGTACAAGGTCTTTTGT TTCTGGATTGGGATCATCGC 45.0 50.0 58.1 57.7 1078-1108 bp 1.0 80.6
petA-psbJ_p2 ACGCGTACAAGGTCTTTTGT TCTGGATTGGGATCATCGCT 45.0 50.0 58.1 58.6 1077-1107 bp 1.0 78.1
petA-psbJ_p3 ACGCGTACAAGGTCTTTTGT CTGGATTGGGATCATCGCTA 45.0 50.0 58.1 56.9 1076-1106 bp 1.0 73.6
petA-psbJ_p4 ACGCGTACAAGGTCTTTTGT ATTCTGGATTGGGATCATCGC 45.0 47.6 58.1 58.2 1079-1109 bp 1.0 73.0
petA-psbJ_p5 CGCGTACAAGGTCTTTTGTT TTCTGGATTGGGATCATCGC 45.0 50.0 57.0 57.7 1077-1107 bp 1.0 72.8