Hotspot

trnM-CAU-atpE

LSC · 110459-110712 · 254 bp

Back to Colobanthus

Pi 0.0135

MarkerSeek score 62.60

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 254
variable_sites: 8
pi: 0.0135
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	TATTGCTTTCATACGGCGGG	CTCTCAGACGAGCTAGGACA	50.0	55.0	58.4	58.0	340-359 bp	1.0	81.7
trnM-CAU-atpE_p2	TATTGCTTTCATACGGCGGG	CGAGCTAGGACACGAGTAGA	50.0	55.0	58.4	58.1	332-351 bp	1.0	81.6
trnM-CAU-atpE_p3	TATTGCTTTCATACGGCGGG	TCTCAGACGAGCTAGGACAC	50.0	55.0	58.4	58.3	339-358 bp	1.0	79.9
trnM-CAU-atpE_p4	GTATTGCTTTCATACGGCGG	CTCTCAGACGAGCTAGGACA	50.0	55.0	57.3	58.0	341-360 bp	1.0	79.7
trnM-CAU-atpE_p5	GTATTGCTTTCATACGGCGG	CGAGCTAGGACACGAGTAGA	50.0	55.0	57.3	58.1	333-352 bp	1.0	79.5