Hotspot

trnG-GCC-trnfM-CAU

LSC · 94231-94430 · 200 bp

Back to Colobanthus

Pi 0.0154

MarkerSeek score 59.60

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 200
variable_sites: 8
pi: 0.0154
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	TGAGAGCACCTTCCTTCTCA	TACATTCGTCCCCAGTCCTT	50.0	50.0	58.0	58.1	394-406 bp	1.0	83.5
trnG-GCC-trnfM-CAU_p2	TGAGAGCACCTTCCTTCTCA	GTTCAAATCCTGTCTCCGCA	50.0	50.0	58.0	58.2	313-325 bp	1.0	83.4
trnG-GCC-trnfM-CAU_p3	TGAGAGCACCTTCCTTCTCA	TTGTTCGTACATTCGTCCCC	50.0	50.0	58.0	57.9	401-413 bp	1.0	83.2
trnG-GCC-trnfM-CAU_p4	TGAGAGCACCTTCCTTCTCA	ATTCGTCCCCAGTCCTTTTG	50.0	50.0	58.0	57.8	391-403 bp	1.0	82.3
trnG-GCC-trnfM-CAU_p5	TGAGAGCACCTTCCTTCTCA	CATTCGTCCCCAGTCCTTTT	50.0	50.0	58.0	57.8	392-404 bp	1.0	82.3