Hotspot

psbM-trnD-GUC

LSC · 87462-88176 · 715 bp

Back to Colobanthus

Pi 0.0115

MarkerSeek score 54.70

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 715
variable_sites: 21
pi: 0.0115
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAATAGCGCAGTAGCA	TCAATTGGTTAGAGCACCGC	42.9	50.0	57.5	58.6	816-829 bp	1.0	67.5
psbM-trnD-GUC_p2	GAATGAATAGCGCAGTAGCA	TCAATTGGTTAGAGCACCGC	45.0	50.0	56.0	58.6	815-828 bp	1.0	63.4
psbM-trnD-GUC_p3	AGAATGAATAGCGCAGTAGCA	AATTGGTTAGAGCACCGCC	42.9	52.6	57.5	58.1	814-827 bp	1.0	62.9
psbM-trnD-GUC_p4	AGAATGAATAGCGCAGTAGCAA	TCAATTGGTTAGAGCACCGC	40.9	50.0	58.2	58.6	816-829 bp	1.0	61.6
psbM-trnD-GUC_p5	GAATGAATAGCGCAGTAGCAA	TCAATTGGTTAGAGCACCGC	42.9	50.0	56.8	58.6	815-828 bp	1.0	61.4