Hotspot

ycf1

SSC · 122490-128150 · 5661 bp

Back to Collinsonia

Pi 0.00836

MarkerSeek score 44.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5661
variable_sites: 71
pi: 0.00836
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGAATTGGATTGACTTTTGAAAG	AAACCCATTTTAAAGGAATTCA	30.4	27.3	53.8	52.4	191 bp	1.0	43.4
ycf1_p2	AGAATTGGATTGACTTTTGAAAG	AACCCATTTTAAAGGAATTCAA	30.4	27.3	53.8	52.4	190 bp	1.0	43.4
ycf1_p3	AGAATTGGATTGACTTTTGAAAG	ACCCATTTTAAAGGAATTCAAA	30.4	27.3	53.8	52.4	189 bp	1.0	43.4
ycf1_p4	AGAATTGGATTGACTTTTGAAAG	AACCCATTTTAAAGGAATTCAAA	30.4	26.1	53.8	53.3	190 bp	1.0	43.4
ycf1_p5	AGAATTGGATTGACTTTTGAAAG	ACCCATTTTAAAGGAATTCAAAA	30.4	26.1	53.8	53.3	189 bp	1.0	43.4