Hotspot

petA

LSC · 62212-63174 · 963 bp

Back to Collinsonia

Pi 0.00485

MarkerSeek score 61.80

Primer available yes

Label petA

Feature Metadata

label_name: petA
feature_type: gene
region: LSC
length_bp: 963
variable_sites: 7
pi: 0.00485
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA_p1	AGCAGAATCGTGGATAGCAA	CCTGATGTTGATAAATCCGCG	45.0	47.6	57.0	57.5	1064 bp	1.0	60.4
petA_p2	AGCAGAATCGTGGATAGCAAA	CCTGATGTTGATAAATCCGCG	42.9	47.6	57.7	57.5	1064 bp	1.0	58.2
petA_p3	GCAGAATCGTGGATAGCAAAC	CCTGATGTTGATAAATCCGCG	47.6	47.6	57.7	57.5	1063 bp	1.0	57.7
petA_p4	AGCAGAATCGTGGATAGCAA	TGATGTTGATAAATCCGCGAGT	45.0	40.9	57.0	58.2	1062 bp	1.0	55.0
petA_p5	ACTAGCGACCTACCCAATTT	CCTGATGTTGATAAATCCGCG	45.0	47.6	56.2	57.5	1039 bp	1.0	54.2