Hotspot

rps15-ycf1

SSC · 122090-122489 · 400 bp

Back to Collinsonia

Pi 0.02

MarkerSeek score 61.30

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 400
variable_sites: 12
pi: 0.02
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	ACTTGAAATTCAACAGACCCTCT	TCCGCGATTAAAATTCTGCG	39.1	45.0	57.9	57.3	502-503 bp	1.0	51.6
rps15-ycf1_p2	ACAGACCCTCTATTTTCTTCGT	TCCGCGATTAAAATTCTGCG	40.9	45.0	56.8	57.3	490-491 bp	1.0	50.8
rps15-ycf1_p3	ACTTGAAATTCAACAGACCCTC	TCCGCGATTAAAATTCTGCG	40.9	45.0	56.5	57.3	502-503 bp	1.0	48.4
rps15-ycf1_p4	AACAGACCCTCTATTTTCTTCGT	TCCGCGATTAAAATTCTGCG	39.1	45.0	57.5	57.3	491-492 bp	1.0	48.2
rps15-ycf1_p5	ACAGACCCTCTATTTTCTTCGTT	TCCGCGATTAAAATTCTGCG	39.1	45.0	57.5	57.3	490-491 bp	1.0	48.2