Hotspot

ndhF-rpl32

SSC · 111626-112044 · 419 bp

Back to Collinsonia

Pi 0.0191
MarkerSeek score 57.90
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
419
variable_sites
12
pi
0.0191
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTGGAAGTGGAACGAAAGGT AGCCTTTCCTTTTCCAAAAA 50.0 35.0 57.7 53.7 544-548 bp 1.0 45.8
ndhF-rpl32_p2 CTGGAAGTGGAACGAAAGGT TGATGCAGAAGTACGTTTTT 50.0 35.0 57.7 53.5 504-508 bp 1.0 43.9
ndhF-rpl32_p3 CTGGAAGTGGAACGAAAGGT TTGATGCAGAAGTACGTTTT 50.0 35.0 57.7 53.5 505-509 bp 1.0 43.9
ndhF-rpl32_p4 CTGGAAGTGGAACGAAAGGT TTTGATGCAGAAGTACGTTT 50.0 35.0 57.7 53.5 506-510 bp 1.0 43.9
ndhF-rpl32_p5 CTGGAAGTGGAACGAAAGGT TTTTGATGCAGAAGTACGTT 50.0 35.0 57.7 53.5 507-511 bp 1.0 43.9