Hotspot

rpl20-rps12

LSC · 68866-69675 · 810 bp

Back to Collinsonia

Pi 0.00906
MarkerSeek score 65.10
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
810
variable_sites
11
pi
0.00906
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 ACCCTCGAAAACTTGATGCA CGCCGAGGAACATGTACTAG 45.0 55.0 57.7 57.9 926-929 bp 1.0 78.2
rpl20-rps12_p2 CGGGCTATATATCCGCGTTT CGCCGAGGAACATGTACTAG 50.0 55.0 57.7 57.9 879-882 bp 1.0 78.1
rpl20-rps12_p3 ACCCTCGAAAACTTGATGCA ACTAGGGTGTATGTGCGACT 45.0 50.0 57.7 58.2 911-914 bp 1.0 77.9
rpl20-rps12_p4 CGGGCTATATATCCGCGTTT ACTAGGGTGTATGTGCGACT 50.0 50.0 57.7 58.2 864-867 bp 1.0 77.9
rpl20-rps12_p5 ACCCTCGAAAACTTGATGCA AATACAAGACAGCCCGTTCG 45.0 50.0 57.7 58.3 986-989 bp 1.0 76.8