Hotspot

trnC-GCA-petN

LSC · 28255-28415 · 161 bp

Back to Collinsonia

Pi 0.0124

MarkerSeek score 63.80

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 161
variable_sites: 3
pi: 0.0124
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	ACTTCTTCCCCACACTACGA	50.0	50.0	58.1	58.0	269-277 bp	1.0	82.5
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	TTCTTCCCCACACTACGAGT	50.0	50.0	58.1	58.0	267-275 bp	1.0	82.5
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	CACTTCTTCCCCACACTACG	50.0	55.0	58.1	57.9	270-278 bp	1.0	81.9
trnC-GCA-petN_p4	GTATCATTTTGGCGGCATGG	TTCTTCCCCACACTACGAGT	50.0	50.0	57.9	58.0	319-327 bp	1.0	81.6
trnC-GCA-petN_p5	GTATCATTTTGGCGGCATGG	ACTTCTTCCCCACACTACGA	50.0	50.0	57.9	58.0	321-329 bp	1.0	81.5