Hotspot

rps16-trnQ-UUG

LSC · 5996-7054 · 1059 bp

Back to Collinsonia

Pi 0.0123

MarkerSeek score 61.40

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1059
variable_sites: 19
pi: 0.0123
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GTCTTTCAAGTCGCACGTTG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1097-1139 bp	1.0	84.7
rps16-trnQ-UUG_p2	GTCTTTCAAGTCGCACGTTG	GTCTCGCTATTCGGAGGTTC	50.0	55.0	58.0	57.9	1110-1152 bp	1.0	84.3
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1090-1132 bp	1.0	83.6
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	GTCTCGCTATTCGGAGGTTC	45.0	55.0	58.1	57.9	1103-1145 bp	1.0	83.3
rps16-trnQ-UUG_p5	GTCTTTCAAGTCGCACGTTG	CGGGTTTTGGTCTCGCTATT	50.0	50.0	58.0	58.3	1119-1161 bp	1.0	83.2