Hotspot

ycf1

SSC · 126897-132245 · 5349 bp

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Pi 0.343

MarkerSeek score 73.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5349
variable_sites: 1467
pi: 0.343
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACATACTGAAACGACTGCTGT	CCATTTCACGACACAGCAAC	42.9	50.0	57.6	58.0	342 bp	0.5	48.6
ycf1_p2	ACATACTGAAACGACTGCTGT	CGAACAACAGGTCCAAAGGA	42.9	50.0	57.6	58.0	848 bp	0.5	48.6
ycf1_p3	ACATACTGAAACGACTGCTGT	ATCTCCCAGACGTCGTAGAG	42.9	55.0	57.6	58.0	266 bp	0.5	48.6
ycf1_p4	ACATACTGAAACGACTGCTGT	CTCCCAGACGTCGTAGAGAA	42.9	55.0	57.6	58.3	264 bp	0.5	46.8
ycf1_p5	TGACATACTGAAACGACTGCT	CCATTTCACGACACAGCAAC	42.9	50.0	57.3	58.0	344 bp	0.5	46.4