Hotspot

rpl32

SSC · 116078-116251 · 174 bp

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Pi 0.406

MarkerSeek score 74.90

Primer available yes

Label rpl32

Feature Metadata

label_name: rpl32
feature_type: gene
region: SSC
length_bp: 174
variable_sites: 69
pi: 0.406
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32_p1	ACAACGATAAAAAGGAGTCACC	ACCCATTTGAGACCCAACAC	40.9	50.0	56.6	58.0	615 bp	0.5	33.1
rpl32_p2	ACAACGATAAAAAGGAGTCACC	CATGGCGGCTTACCCTAAAT	40.9	50.0	56.6	58.0	908 bp	0.5	33.0
rpl32_p3	ACAACGATAAAAAGGAGTCACC	TCCTAAGAGCAGCGTGTCTA	40.9	50.0	56.6	57.9	938 bp	0.5	32.2
rpl32_p4	ACAACGATAAAAAGGAGTCACC	AATTTCACCATGGCGGCTTA	40.9	45.0	56.6	58.2	916 bp	0.5	31.9
rpl32_p5	ACAACGATAAAAAGGAGTCACC	TTCGTTCTCAGTCGGGGTAT	40.9	50.0	56.6	58.2	666 bp	0.5	31.9