Hotspot

ndhI

SSC · 122076-122579 · 504 bp

Back to Collabium

Pi 0.477

MarkerSeek score 81.30

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 504
variable_sites: 226
pi: 0.477
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	GCAGAATCGATGGGATTGGT	TTTGGAATAGCGATGGGAGC	50.0	50.0	58.0	58.0	1540 bp	0.5	59.5
ndhI_p2	GCAGAATCGATGGGATTGGT	ACCAGAAGCAGAGGAGGAAT	50.0	50.0	58.0	58.0	1351 bp	0.5	59.5
ndhI_p3	GCAGAATCGATGGGATTGGT	GGAGCCGTATGAAGCTTAGG	50.0	55.0	58.0	57.8	1573 bp	0.5	58.5
ndhI_p4	AAGGGTCCAGGAAAGGAACT	TTTGGAATAGCGATGGGAGC	50.0	50.0	58.2	58.0	1384 bp	0.5	58.4
ndhI_p5	AAGGGTCCAGGAAAGGAACT	ACCAGAAGCAGAGGAGGAAT	50.0	50.0	58.2	58.0	1195 bp	0.5	58.4