Hotspot

ndhF

SSC · 112825-115050 · 2226 bp

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Pi 0.466

MarkerSeek score 81.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2226
variable_sites: 939
pi: 0.466
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACAGGAACAAGAGGGATCCA	TGACCAACCTATTGCAACTCT	50.0	42.9	58.0	57.2	2621 bp	0.5	45.3
ndhF_p2	AAGAGGGATCCACCGAAGAA	TGACCAACCTATTGCAACTCT	50.0	42.9	58.0	57.2	2613 bp	0.5	45.3
ndhF_p3	AGAGGGATCCACCGAAGAAA	TGACCAACCTATTGCAACTCT	50.0	42.9	58.0	57.2	2612 bp	0.5	45.3
ndhF_p4	CAAGTTCCACGTTAGCCAGA	TGACCAACCTATTGCAACTCT	50.0	42.9	57.8	57.2	2976 bp	0.5	44.4
ndhF_p5	CACTTGAAAGAGGCACGCTA	TGACCAACCTATTGCAACTCT	50.0	42.9	58.2	57.2	2459 bp	0.5	44.1