Hotspot

ndhD

SSC · 118391-119899 · 1509 bp

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Pi 0.462

MarkerSeek score 80.40

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1509
variable_sites: 583
pi: 0.462
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	AGGCATTCTTTCTGGAGCAG	AGATGTGAATCCGCTTGTCC	50.0	50.0	57.9	58.0	2262 bp	0.5	58.8
ndhD_p2	GGCATTCTTTCTGGAGCAGT	AGATGTGAATCCGCTTGTCC	50.0	50.0	58.2	58.0	2261 bp	0.5	58.4
ndhD_p3	AGGCATTCTTTCTGGAGCAG	CAAAAGCCCGTACTCGAGAA	50.0	50.0	57.9	57.9	2125 bp	0.5	58.4
ndhD_p4	TCTGGAGCAGTATGGGCTAA	AGATGTGAATCCGCTTGTCC	50.0	50.0	57.8	58.0	2252 bp	0.5	58.4
ndhD_p5	TTCTGGAGCAGTATGGGCTA	AGATGTGAATCCGCTTGTCC	50.0	50.0	57.8	58.0	2253 bp	0.5	58.4