Hotspot

ndhA

SSC · 122700-124931 · 2232 bp

Back to Collabium

Pi 0.466

MarkerSeek score 78.70

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2232
variable_sites: 860
pi: 0.466
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TCGACACAGTTACCGCAAAA	TTGCAAATTCTTCCCCAGCT	45.0	45.0	58.1	58.0	2787 bp	0.5	59.4
ndhA_p2	ATTGGACATACGCGAACACA	TTGCAAATTCTTCCCCAGCT	45.0	45.0	57.9	58.0	2679 bp	0.5	59.2
ndhA_p3	TTGGACATACGCGAACACAT	TTGCAAATTCTTCCCCAGCT	45.0	45.0	57.9	58.0	2678 bp	0.5	59.2
ndhA_p4	TCGACACAGTTACCGCAAAA	TAGAGGCCCCAAAAGGAGAA	45.0	50.0	58.1	57.9	2882 bp	0.5	59.1
ndhA_p5	CTTGCGGCTCGTATTGTTTG	TTGCAAATTCTTCCCCAGCT	50.0	45.0	58.1	58.0	2511 bp	0.5	58.9