Hotspot

ndhD-psaC

SSC · 115579-115695 · 117 bp

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Pi 0.0513
MarkerSeek score 76.20
Primer available yes
Label ndhD-psaC

Feature Metadata

label_name
ndhD-psaC
feature_type
igs
region
SSC
length_bp
117
variable_sites
12
pi
0.0513
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD-psaC_p1 AAACAAGGAAAATGGTTCGT GAATCCGCTTGTCCAACAGA 35.0 50.0 53.9 58.2 221 bp 1.0 50.0
ndhD-psaC_p2 AAACAAGGAAAATGGTTCGT AAGAGGTGTGAATCCGCTTG 35.0 50.0 53.9 58.2 230 bp 1.0 49.8
ndhD-psaC_p3 AAACAAGGAAAATGGTTCGT TCCGCTTGTCCAACAGATTT 35.0 45.0 53.9 57.7 218 bp 1.0 49.4
ndhD-psaC_p4 AAACAAGGAAAATGGTTCGT TGTAAGAGGTGTGAATCCGC 35.0 50.0 53.9 57.6 233 bp 1.0 48.2
ndhD-psaC_p5 AAACAAGGAAAATGGTTCGT GCGTTGGTTGTAAGAGGTGT 35.0 50.0 53.9 58.4 241 bp 1.0 47.9