Hotspot

trnL-UAG-ccsA

SSC · 112797-112886 · 90 bp

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Pi 0.0315
MarkerSeek score 69.40
Primer available yes
Label trnL-UAG-ccsA

Feature Metadata

label_name
trnL-UAG-ccsA
feature_type
igs
region
SSC
length_bp
90
variable_sites
5
pi
0.0315
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.94
markerseek_score
69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-ccsA_p1 TAGACACGCTGCTCTTAGGA AGAATTGAAACAGTCGAAAAAGA 50.0 30.4 57.9 55.0 211-217 bp 1.0 44.1
trnL-UAG-ccsA_p2 GGTAGACACGCTGCTCTTAG AGAATTGAAACAGTCGAAAAAGA 55.0 30.4 57.8 55.0 213-219 bp 1.0 44.1
trnL-UAG-ccsA_p3 GTAGACACGCTGCTCTTAGG AGAATTGAAACAGTCGAAAAAGA 55.0 30.4 57.8 55.0 212-218 bp 1.0 44.1
trnL-UAG-ccsA_p4 AAATTGGTAGACACGCTGCT AGAATTGAAACAGTCGAAAAAGA 45.0 30.4 57.8 55.0 218-224 bp 1.0 44.0
trnL-UAG-ccsA_p5 ACAAGCCGCTATGGTGAAAT AGAATTGAAACAGTCGAAAAAGA 45.0 30.4 57.9 55.0 234-240 bp 1.0 43.7