Hotspot

psaA-pafI

LSC · 41269-42052 · 784 bp

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Pi 0.0166
MarkerSeek score 72.50
Primer available yes
Label psaA-pafI

Feature Metadata

label_name
psaA-pafI
feature_type
igs
region
LSC
length_bp
784
variable_sites
24
pi
0.0166
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-pafI_p1 GCCCATTCCTCGAAAGAAGT ATTGGTTGAAGATCACGGGG 50.0 50.0 57.9 57.9 873-900 bp 1.0 83.5
psaA-pafI_p2 GAAAAATGACCCGGTTTGGC ATTGGTTGAAGATCACGGGG 50.0 50.0 58.2 57.9 891-918 bp 1.0 82.7
psaA-pafI_p3 GCCCATTCCTCGAAAGAAGT TGGTTTGATCAAGCTGCTGA 50.0 45.0 57.9 57.7 946-973 bp 1.0 81.9
psaA-pafI_p4 GAAAAATGACCCGGTTTGGC TGGTTTGATCAAGCTGCTGA 50.0 45.0 58.2 57.7 964-991 bp 1.0 81.1
psaA-pafI_p5 GCCCATTCCTCGAAAGAAGT CGGAGGCTTGGTTTGATCAA 50.0 50.0 57.9 58.5 954-981 bp 1.0 80.4