Hotspot

petN-psbM

LSC · 28358-29346 · 989 bp

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Pi 0.0216
MarkerSeek score 71.60
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
989
variable_sites
40
pi
0.0216
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 ACTCGTAGTGTGGGGAAGAA AGTTCCTACCGCTTTTCTACT 50.0 42.9 58.0 56.4 1078-1107 bp 1.0 65.5
petN-psbM_p2 TCGTAGTGTGGGGAAGAAGT AGTTCCTACCGCTTTTCTACT 50.0 42.9 58.0 56.4 1076-1105 bp 1.0 65.5
petN-psbM_p3 ACTCGTAGTGTGGGGAAGAA TCATTCTAGTTCCTACCGCT 50.0 45.0 58.0 55.4 1085-1114 bp 1.0 65.3
petN-psbM_p4 TCGTAGTGTGGGGAAGAAGT TCATTCTAGTTCCTACCGCT 50.0 45.0 58.0 55.4 1083-1112 bp 1.0 65.3
petN-psbM_p5 CGTAGTGTGGGGAAGAAGTG AGTTCCTACCGCTTTTCTACT 55.0 42.9 57.9 56.4 1075-1104 bp 1.0 64.9