Hotspot

atpH-atpI

LSC · 13496-14544 · 1049 bp

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Pi 0.0224
MarkerSeek score 73.70
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1049
variable_sites
44
pi
0.0224
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 AACGGAAGCAGCAGAAATCA TCCCTGTCATGTTCCTTGGA 45.0 50.0 58.1 58.3 1118-1183 bp 1.0 84.1
atpH-atpI_p2 AGCCAATCCAGCAGCAATAA TCCCTGTCATGTTCCTTGGA 45.0 50.0 57.9 58.3 1136-1201 bp 1.0 83.7
atpH-atpI_p3 GCCAATCCAGCAGCAATAAC TCCCTGTCATGTTCCTTGGA 50.0 50.0 57.8 58.3 1135-1200 bp 1.0 83.2
atpH-atpI_p4 TCCAGCAGCAATAACGGAAG TCCCTGTCATGTTCCTTGGA 50.0 50.0 58.3 58.3 1130-1195 bp 1.0 82.8
atpH-atpI_p5 AACGGAAGCAGCAGAAATCA TTTTTGCAACTTTAGCCGCG 45.0 45.0 58.1 58.5 1070-1135 bp 1.0 82.2