Hotspot

psbI-trnS-GCU

LSC · 7972-8116 · 145 bp

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Pi 0.0448
MarkerSeek score 74.20
Primer available yes
Label psbI-trnS-GCU

Feature Metadata

label_name
psbI-trnS-GCU
feature_type
igs
region
LSC
length_bp
145
variable_sites
12
pi
0.0448
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-GCU_p1 GCGTAATCCTGGACGTGAAG TTATTCGTACCGAGGGTTCG 55.0 50.0 58.7 57.2 169-225 bp 1.0 76.1
psbI-trnS-GCU_p2 GCGTAATCCTGGACGTGAAG TTCGAATCCCTCTCTTTCCG 55.0 50.0 58.7 57.0 153-209 bp 1.0 75.6
psbI-trnS-GCU_p3 GCGTAATCCTGGACGTGAAG GGGTTCGAATCCCTCTCTTT 55.0 50.0 58.7 56.9 156-212 bp 1.0 74.6
psbI-trnS-GCU_p4 GCGTAATCCTGGACGTGAAG GGTTCGAATCCCTCTCTTTCC 55.0 52.4 58.7 58.1 155-211 bp 1.0 74.5
psbI-trnS-GCU_p5 CGTAATCCTGGACGTGAAGA TTATTCGTACCGAGGGTTCG 50.0 50.0 57.1 57.2 168-224 bp 1.0 74.4