Hotspot

rps16-trnQ-UUG

LSC · 5915-6908 · 994 bp

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Pi 0.0206
MarkerSeek score 67.90
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
994
variable_sites
37
pi
0.0206
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.96
markerseek_score
67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TCCACAACGGGTCATATCCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.1 58.1 1038-1124 bp 1.0 85.1
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1016-1102 bp 1.0 85.1
rps16-trnQ-UUG_p3 CCACAACGGGTCATATCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.3 58.1 1037-1123 bp 1.0 80.5
rps16-trnQ-UUG_p4 TCCACAACGGGTCATATCCT TTCGGAGGTTCGAATCCTTC 50.0 50.0 58.1 57.3 1042-1128 bp 1.0 80.4
rps16-trnQ-UUG_p5 TCCACAACGGGTCATATCCT TAAGGCAACGGGTTTTGGTC 50.0 50.0 58.1 58.7 1068-1154 bp 1.0 80.2