Hotspot

accD-tRNA-Arg

Genome · 108333-108774 · 442 bp

Back to Coleochaete

Pi 0.0348

MarkerSeek score 71.90

Primer available yes

Label accD-tRNA-Arg

Feature Metadata

label_name: accD-tRNA-Arg
feature_type: igs
region: Genome
length_bp: 442
variable_sites: 15
pi: 0.0348
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-tRNA-Arg_p1	CAAACCAGTCAACCAAAGACA	AGGCTTGTAGCTCAGAGGAT	42.9	50.0	56.9	57.8	536-539 bp	1.0	65.4
accD-tRNA-Arg_p2	CAAACCAGTCAACCAAAGACA	GTGTTGAGGCTTGTAGCTCA	42.9	50.0	56.9	57.8	542-545 bp	1.0	65.3
accD-tRNA-Arg_p3	CAAACCAGTCAACCAAAGACA	TGTGTTGAGGCTTGTAGCTC	42.9	50.0	56.9	57.8	543-546 bp	1.0	65.3
accD-tRNA-Arg_p4	CAAACCAGTCAACCAAAGACA	GAGGATAAAGAGCGCATGGT	42.9	50.0	56.9	57.8	522-525 bp	1.0	64.9
accD-tRNA-Arg_p5	TCAAACCAGTCAACCAAAGACA	GTGTTGAGGCTTGTAGCTCA	40.9	50.0	58.3	57.8	543-546 bp	1.0	64.0